Monday, 15 September 2014

Anthelmintic Screening of Crude Extracts of Hygrocybe Parvula

Anthelmintic Screening of Crude Extracts of Hygrocybe Parvula: The World Health Organization estimates that a staggering two billion people harbor parasitic worm infections. Parasitic worms also infect livestock and crops, affecting food production with a resultant economic impact. Despite this prevalence of parasitic infections, the research on anthelmintic drug is poor. As per WHO, only few drugs are frequently used in the treatment of helminthes in human beings. Anthelmintic from the natural sources may play a key role in the treatment of this parasitic infections.In the present investigation, anthelmintic screening of crude extracts of Hygrocybe parvula. The present study aimed at the in-vitro evaluation of anthelmintic activity of various solvent extracts of fruiting bodies of H. parvula using at three different concentrations (25, 50 and 100mg/ml) respectively. The study involved the determination of time of paralysis (P) and time of death (D) of the worms. In view of this petroleum ether, chloroform and methanol extracts of fruiting bodies of H. parvula were evaluated separately for the activity on adult Indian earthworms, Pheretima posthuma, using Piperazine citrate as reference standards. The results indicated that the petroleum ether extract was more potent than the chloroform and methanol solvent extract. All extracts showed effective anthelmintic activity at 100mg/ml concentration.